Changes in memory function of rats with brain injury after fingolimod administration combined with bone marrow mesenchymal stem cell transplantation

doi:10.3969/j.issn.2095-4344.2014.28.007

Abstract

Abstract:

BACKGROUND: The repair effects of bone marrow mesenchymal stem cell transplantation on brain injury were not ideal. Combined therapy with medicine and biological engineering materials is needed.
OBJECTIVE: To investigate the effects of bone marrow mesenchymal stem cell transplantation and fingolimod immunosuppressants on memory function recovery in rats with brain injury.
METHODS: A total of 60 healthy Sprague-Dawley rats were subjected to hydraulic shock with peak value of 253.312 5–303.975 kPa with a hydraulic head injury instrument so as to induce a model of severe hydraulic head injury. They were randomly divided into brain injury group, bone marrow mesenchymal stem cell transplantation group and fingolimod + bone marrow mesenchymal stem cell transplantation group. The Morris water maze test was tested at 21–28 days after PKH-26-labeled bone marrow mesenchymal stem cell transplantation. The PKH-26 immunofluorescence and hematoxylin-eosin staining were conducted in brain tissues at 4 weeks after brain injury.
RESULTS AND CONCLUSION: At 4 weeks after transplantation, the average escape latency was gradually decreased in each group. The average escape latency was shorter in the fingolimod + bone marrow mesenchymal stem cell transplantation group than in the bone marrow mesenchymal stem cell transplantation group (P < 0.05), and significantly shorter than in the brain injury group (P < 0.01). The number of times of crossing the platform and the percentage of swimming distance to total distance were higher in the fingolimod + bone marrow mesenchymal stem cell transplantation group than in the brain injury group and bone marrow mesenchymal stem cell transplantation group (P < 0.05). The number of PKH-26-positive cells was significantly higher in the fingolimod + bone marrow mesenchymal stem cell transplantation group than in the brain injury group and bone marrow mesenchymal stem cell transplantation group (P < 0.05). Results confirmed that bone marrow mesenchymal stem cell transplantation could apparently improve memory function of rats with severe brain injury. The combined application of fingolimod immunosuppressants has synergistic effects.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: bone marrow, mesenchymal stem cells, brain injuries, immunosuppressive agents

CLC Number:

R394.2

Wang Cheng. Changes in memory function of rats with brain injury after fingolimod administration combined with bone marrow mesenchymal stem cell transplantation[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(28): 4469-4473.

Figures/Tables 4

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